The antiserum was produced against synthesized peptide derived from human Keratin 8 around the phosphorylation site of Ser73. AA range:41-90
Alternative Names:
KRT8, CYK8, Keratin, type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8
keratin 8(KRT8) Homo sapiens This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012],
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Western Blot analysis of various cells using Phospho-Cytokeratin 8 (S73) Polyclonal Antibody diluted at 1:500
Western Blot analysis of 293 cells using Phospho-Cytokeratin 8 (S73) Polyclonal Antibody diluted at 1:500
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Keratin 8 (Phospho-Ser73) Antibody
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