The antiserum was produced against synthesized peptide derived from human DARPP-32 around the phosphorylation site of Thr75. AA range:41-90
Alternative Names:
PPP1R1B, DARPP32, Protein phosphatase 1 regulatory subunit 1B, DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein
This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011],
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Western Blot analysis of cell lysis treated or untreated by LPS 100ng/mL 30min, using primary antibody at 1:1000 dilution. Secondary antibody(catalog:RS23920) was diluted at 1:10000
Western Blot analysis of various cells using Phospho-DARPP-32 (T75) Polyclonal Antibody diluted at 1:500
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4 overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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