EGFR (phospho Tyr1197) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: EGFR (phospho Tyr1197) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1303
• Supplier Catalog Number: ES1303
• Alternative Catalog Number: EBT-ES1303-50, EBT-ES1303-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human EGFR around the phosphorylation site of Tyr1197. AA range:1161-1210
• Alternative Names: EGFR, ERBB, ERBB1, HER1, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

Product Description

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 175kD
• NCBI: 1956
• UniProt: P00533

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Images

Western Blot analysis of various cells using Phospho-EGFR (Y1197) Polyclonal Antibody diluted at 1:1000

Western Blot analysis of A431 cells using Phospho-EGFR (Y1197) Polyclonal Antibody diluted at 1:1000

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using EGFR (Phospho-Tyr1197) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from A431 cells treated with EGF 40 muM 10, using EGFR (Phospho-Tyr1197) Antibody. The lane on the left is blocked with the phospho peptide.