HSP27 (phospho Ser82) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: HSP27 (phospho Ser82) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1332
• Supplier Catalog Number: ES1332
• Alternative Catalog Number: EBT-ES1332-50, EBT-ES1332-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human HSP27 around the phosphorylation site of Ser82. AA range:48-97
• Alternative Names: HSPB1, HSP27, HSP28, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27, SRP27

Product Description

The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 27kD
• NCBI: 3315
• UniProt: P04792

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Images

Western Blot analysis of various cells using Phospho-HSP27 (S82) Polyclonal Antibody diluted at 1:1000

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HSP27 (Phospho-Ser82) Antibody

Western blot analysis of lysates from HUVEC cells treated with TNF 20ng/ml 30, using HSP27 (Phospho-Ser82) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4 overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).