Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Western blot analysis of lysates from 1) Hela , 2)3T3, 3) Jurkat cells, (Green) primary antibody was diluted at 1:1000, 4over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37 1hour. (Red) Actin beta Monoclonal Antibody(5B7) (cat:YM3028) antibody was diluted at 1:5000 as loading control, 4 over night,secondary antibody(cat:RS23710)was diluted at 1:10000, 37 1hour.
Western Blot analysis of various cells using Phospho-Integrin beta1 (T788) Polyclonal Antibody
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Integrin beta1 (Phospho-Thr788) Antibody
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Integrin beta1 (Phospho-Thr788) Antibody. The picture on the right is blocked with the phospho peptide.
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