NFkappaB-p65 (phospho Ser529) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: NFkappaB-p65 (phospho Ser529) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1370
• Supplier Catalog Number: ES1370
• Alternative Catalog Number: EBT-ES1370-50, EBT-ES1370-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Monkey, Mouse, Rat
• Immunogen: Synthesized phospho-peptide around the phosphorylation site of human NFkappaB-p65 (phospho Ser529)
• Alternative Names: RELA, NFKB3, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

Product Description

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 60kD
• NCBI: 5970
• UniProt: Q04206

Application Notes

• Application Dilute: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Images

Immunofluorescence analysis of human-lung tissue. 1,NFkappaB-p65 (phospho Ser529) Polyclonal Antibody(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of human-lung tissue. 1,NFkappaB-p65 (phospho Ser529) Polyclonal Antibody(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of rat-heart tissue. 1,NFkappaB-p65 (phospho Ser529) Polyclonal Antibody(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of rat-heart tissue. 1,NFkappaB-p65 (phospho Ser529) Polyclonal Antibody(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B