p70 S6 kinase alpha (phospho Thr229) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: p70 S6 kinase alpha (phospho Thr229) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1390
• Supplier Catalog Number: ES1390
• Alternative Catalog Number: EBT-ES1390-50, EBT-ES1390-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human p70 S6 Kinase around the phosphorylation site of Thr229. AA range:195-244
• Alternative Names: RPS6KB1, STK14A, Ribosomal protein S6 kinase beta-1, S6K-beta-1, S6K1, 70 kDa ribosomal protein S6 kinase 1, P70S6K1, p70-S6K 1, Ribosomal protein S6 kinase I, Serine/threonine-protein kinase 14A, p70 ribosomal S6 kinase alpha, p70 S6 kinas

Product Description

ribosomal protein S6 kinase B1(RPS6KB1) Homo sapiens This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 60kD
• NCBI: 6198
• UniProt: P23443

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

Images

Immunofluorescence analysis of Hela cell. 1,p70 S6 kinase alpha (phospho Thr229) Polyclonal Antibody(red) was diluted at 1:200(4 overnight). Caspase-8 Monoclonal Antibody(2G12)(green) was diluted at 1:200(4 overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).

Western Blot analysis of various cells using Phospho-p70 S6 kinase alpha (T229) Polyclonal Antibody diluted at 1:500

Western Blot analysis of LOVO cells using Phospho-p70 S6 kinase alpha (T229) Polyclonal Antibody diluted at 1:500

Immunohistochemistry analysis of paraffin-embedded human brain, using p70 S6 Kinase (Phospho-Thr229) Antibody. The picture on the right is blocked with the phospho peptide.