PLC gamma1 (phospho Tyr783) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: PLC gamma1 (phospho Tyr783) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1396
• Supplier Catalog Number: ES1396
• Alternative Catalog Number: EBT-ES1396-50, EBT-ES1396-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Monkey, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human PLCG1 around the phosphorylation site of Tyr783. AA range:751-800
• Alternative Names: PLCG1, PLC1, 1-phosphatidylinositol 4, 5-bisphosphate phosphodiesterase gamma-1, PLC-148, Phosphoinositide phospholipase C-gamma-1, Phospholipase C-II, PLC-II, Phospholipase C-gamma-1, PLC-gamma-1

Product Description

The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 150kD
• NCBI: 5335
• UniProt: P19174

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Images

Western Blot analysis of various cells using Phospho-PLC gamma1 (Y783) Polyclonal Antibody

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PLCG1 (Phospho-Tyr783) Antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PLCG1 (Phospho-Tyr783) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30, using PLCG1 (Phospho-Tyr783) Antibody. The lane on the right is blocked with the phospho peptide.