Rb (phospho Ser780) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: Rb (phospho Ser780) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1400
• Supplier Catalog Number: ES1400
• Alternative Catalog Number: EBT-ES1400-50, EBT-ES1400-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human Retinoblastoma around the phosphorylation site of Ser780. AA range:751-800
• Alternative Names: RB1, Retinoblastoma-associated protein, p105-Rb, pRb, Rb, pp110

Product Description

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 106kD
• NCBI: 5925
• UniProt: P06400

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Images

Immunofluorescence analysis of Hela cell. 1,Rb (phospho Ser780) Polyclonal Antibody(red) was diluted at 1:200(4 overnight). GFAP Monoclonal Antibody(5C8)(green) was diluted at 1:200(4 overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).

Western Blot analysis of various cells using Phospho-Rb (S780) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

Western Blot analysis of KB cells using Phospho-Rb (S780) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Retinoblastoma (Phospho-Ser780) Antibody. The picture on the right is blocked with the phospho peptide.