Shb (phospho Tyr246) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: Shb (phospho Tyr246) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1402
• Supplier Catalog Number: ES1402
• Alternative Catalog Number: EBT-ES1402-50, EBT-ES1402-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Human, Mouse
• Immunogen: Synthesized phospho-peptide around the phosphorylation site of human Shb (phospho Tyr246)
• Alternative Names: SHB, SH2 domain-containing adapter protein B

Product Description

domain:The SH2 domain preferentially binds phosphopeptides with the consensus sequence Y-[TVI]-X-L and mediates interaction with PDGFRA, PDGFRB, FGRFR1, IL2RB, IL2RG, CD3Z and CRK/CrKII.,function:Adapter protein which regulates several signal transduction cascades by linking activated receptors to downstream signaling components. May play a role in angiogenesis by regulating FGFR1, VEGFR2 and PDGFR signaling. May also play a role in T-cell antigen receptor/TCR signaling, interleukin-2 signaling, apoptosis and neuronal cells differentiation by mediating basic-FGF and NGF-induced signaling cascades. May also regulate IRS1 and IRS2 signaling in insulin-producing cells.,PTM:Phosphorylated upon PDGFRA, PDGFRB, TCR, IL2 receptor, FGRF1 or VEGFR2 activation.,similarity:Contains 1 SH2 domain.,subcellular location:Associates with membrane lipid rafts upon TCR stimulation.,subunit:Interacts with PTPN11 (By similarity). Interacts with phosphorylated Tyr-720 of the ligand-activated receptor PDGFRA via its SH2 domain. Interacts with the ligand-activated receptors PDGFRB, FGFR1, KDR/VEGFR2, IL2RB and IL2RG. Interacts with EPS8 and V-SRC. Interacts with GRB2 and GRAP. Interacts with CD3Z. Interacts with tyrosine-phosphorylated LAT upon T-cell antigen receptor activation. Interacts with PLCG1. Interacts with ZAP70, LCP2/SLP-76, VAV1 and GRAP2. Interacts with JAK1 and JAK3. Interacts with PTK2/FAK1. Interacts with CRK/CrKII. Interacts with IRS2.,tissue specificity:Widely expressed.,

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 55kD
• NCBI: 6461
• UniProt: Q15464

Application Notes

• Application Dilute: WB 1:500-2000,IHC-p 1:50-300

Images

Western Blot analysis of various cells using Phospho-Shb (Y246) Polyclonal Antibody

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Shb (Phospho-Tyr246) Antibody

Western blot analysis of lysate from 293 cells, using phospho-Serhb (Phospho-Tyr246) antibody.

Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4 overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).