The antiserum was produced against synthesized peptide derived from human STAT1 around the phosphorylation site of Tyr701. AA range:668-717
Alternative Names:
STAT1, Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008],
IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications.
Immunofluorescence analysis of Hela cell. 1,Stat1 (phospho Tyr701) Polyclonal Antibody(red) was diluted at 1:200(4 overnight). HER2 Monoclonal Antibody(11H9)(green) was diluted at 1:200(4 overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
Western blot analysis of lysates from 1) MOUSE-BRAIN, 2) SY-SY5X ,3) COS7 cells, (Green) primary antibody was diluted at 1:1000, 4over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37 1hour. (Red) Actin beta Monoclonal Antibody(5B7) (cat:YM3028) antibody was diluted at 1:5000 as loading control, 4 over night,secondary antibody(cat:RS23710)was diluted at 1:10000, 37 1hour.
Immunofluorescence analysis of rat-spleen tissue. 1,Stat1 (phospho Tyr701) Polyclonal Antibody(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-spleen tissue. 1,Stat1 (phospho Tyr701) Polyclonal Antibody(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
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