NFkappaB-p105/p50 (phospho Ser337) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: NFkappaB-p105/p50 (phospho Ser337) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1466
• Supplier Catalog Number: ES1466
• Alternative Catalog Number: EBT-ES1466-50, EBT-ES1466-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse
• Immunogen: Synthesized phospho-peptide around the phosphorylation site of human NFkappaB-p105/p50 (phospho Ser337)
• Alternative Names: NFKB1, Nuclear factor NF-kappa-B p105 subunit, DNA-binding factor KBF1, EBP-1, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

Product Description

nuclear factor kappa B subunit 1(NFKB1) Homo sapiens This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isof

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 105+50kD
• NCBI: 4790
• UniProt: P19838

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Images

Western Blot analysis of various cells using Phospho-NFkappaB-p105/p50 (S337) Polyclonal Antibody diluted at 1:500

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemistry analysis of paraffin-embedded human breast cancer, using NF-kappaB p105/p50 (Phospho-Ser337) Antibody. The picture on the right is blocked with the NF-kappaB p105/p50 (Phospho-Ser337) peptide.