C/EBP alpha (phospho Ser21) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: C/EBP alpha (phospho Ser21) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1468
• Supplier Catalog Number: ES1468
• Alternative Catalog Number: EBT-ES1468-50, EBT-ES1468-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human C/EBP-alpha around the phosphorylation site of Ser21. AA range:6-55
• Alternative Names: CEBPA, CCAAT/enhancer-binding protein alpha, C/EBP alpha

Product Description

This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 42kd
• NCBI: 1050
• UniProt: P49715

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Images

Western blot analysis of lysates from Mouse-liver, Rat-liver, Hela cells treated with 0.01U/ml 30min, using C/EBP alpha (phospho Ser21) Polyclonal Antibody. Primary Antibody was diluted at 1:1000 4 over night,secondary antibody(Immunoway cat:RS23710)was diluted at 1:10000, 37 1hour.

Western Blot analysis of various cells using Phospho-C/EBP alpha (S21) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4 overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using C/EBP-alpha (Phospho-Ser21) Antibody. The picture on the right is blocked with the phospho peptide.