PLC beta3 (phospho Ser1105) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: PLC beta3 (phospho Ser1105) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1475
• Supplier Catalog Number: ES1475
• Alternative Catalog Number: EBT-ES1475-50, EBT-ES1475-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human PLCB3 around the phosphorylation site of Ser1105. AA range:1071-1120
• Alternative Names: PLCB3, 1-phosphatidylinositol 4, 5-bisphosphate phosphodiesterase beta-3, Phosphoinositide phospholipase C-beta-3, Phospholipase C-beta-3, PLC-beta-3

Product Description

This gene encodes a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 160kD
• NCBI: 5331
• UniProt: Q01970

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Images

Western Blot analysis of various cells using Phospho-PLC beta3 (S1105) Polyclonal Antibody diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PLCB3 (Phospho-Ser1105) Antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using PLCB3 (Phospho-Ser1105) Antibody. The picture on the right is blocked with the phospho peptide.