PLC gamma2 (phospho Tyr753) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: PLC gamma2 (phospho Tyr753) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1477
• Supplier Catalog Number: ES1477
• Alternative Catalog Number: EBT-ES1477-50, EBT-ES1477-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human PLCG2 around the phosphorylation site of Tyr753. AA range:721-770
• Alternative Names: PLCG2, 1-phosphatidylinositol 4, 5-bisphosphate phosphodiesterase gamma-2, Phosphoinositide phospholipase C-gamma-2, Phospholipase C-IV, PLC-IV, Phospholipase C-gamma-2, PLC-gamma-2

Product Description

The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3. [provided by RefSeq, Mar 2014],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 148kD
• NCBI: 5336
• UniProt: P16885

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Images

Western Blot analysis of various cells using Phospho-PLC gamma2 (Y753) Polyclonal Antibody

Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4 overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PLCG2 (Phospho-Tyr753) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HepG2 cells treated with Na3VO4 0.3mM 40, using PLCG2 (Phospho-Tyr753) Antibody. The lane on the right is blocked with the phospho peptide.