PFK-2 car (phospho Ser483) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: PFK-2 car (phospho Ser483) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1490
• Supplier Catalog Number: ES1490
• Alternative Catalog Number: EBT-ES1490-50, EBT-ES1490-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human PFKFB2 around the phosphorylation site of Ser483. AA range:451-500
• Alternative Names: PFKFB2, 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 2, 6PF-2-K/Fru-2,6-P2ase 2, PFK/FBPase 2, 6PF-2-K/Fru-2,6-P2ase heart-type isozyme

Product Description

The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 58kD
• NCBI: 5208
• UniProt: O60825

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Images

Western Blot analysis of various cells using Phospho-PFK-2 car (S483) Polyclonal Antibody diluted at 1:1000

Western Blot analysis of Hela cells using Phospho-PFK-2 car (S483) Polyclonal Antibody diluted at 1:1000

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PFKFB2 (Phospho-Ser483) Antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using PFKFB2 (Phospho-Ser483) Antibody. The picture on the right is blocked with the phospho peptide.