Cortactin (phospho Tyr466) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: Cortactin (phospho Tyr466) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1500
• Supplier Catalog Number: ES1500
• Alternative Catalog Number: EBT-ES1500-50, EBT-ES1500-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Monkey, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from mouse Cortactin around the phosphorylation site of Tyr466. AA range:441-490
• Alternative Names: CTTN, EMS1, Src substrate cortactin, Amplaxin, Oncogene EMS1

Product Description

cortactin(CTTN) Homo sapiens This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq, May 2010],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 80kD
• NCBI: 13043
• UniProt: Q14247

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Images

Immunofluorescence analysis of Hela cell. 1,Cortactin (phospho Tyr466) Polyclonal Antibody(green) was diluted at 1:200(4 overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.

Western Blot analysis of various cells using Phospho-Cortactin (Y466) Polyclonal Antibody diluted at 1:500

Western Blot analysis of 293 cells using Phospho-Cortactin (Y466) Polyclonal Antibody diluted at 1:500

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Cortactin (Phospho-Tyr466) Antibody. The picture on the right is blocked with the phospho peptide.