Adducin alpha/beta (phospho Ser726/713) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: Adducin alpha/beta (phospho Ser726/713) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1504
• Supplier Catalog Number: ES1504
• Alternative Catalog Number: EBT-ES1504-50, EBT-ES1504-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human ADD1 around the phosphorylation site of Ser726. AA range:688-737
• Alternative Names: ADD1, ADDA, Alpha-adducin, Erythrocyte adducin subunit alpha, ADD2, ADDB, Beta-adducin, Erythrocyte adducin subunit beta

Product Description

adducin 1(ADD1) Homo sapiens Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms, however, not all variants have been fully described. [provided by RefSeq, Jul 2008],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 80kD
• NCBI: 118
• UniProt: P35611

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

Images

Western Blot analysis of various cells using Phospho-Adducin alpha/beta (S726/713) Polyclonal Antibody diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using ADD1 (Phospho-Ser726) Antibody

Immunofluorescence analysis of HeLa cells, using ADD1 (Phospho-Ser726) Antibody. The picture on the right is blocked with the phospho peptide.