HSP90beta (phospho Ser254) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: HSP90beta (phospho Ser254) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1507
• Supplier Catalog Number: ES1507
• Alternative Catalog Number: EBT-ES1507-50, EBT-ES1507-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Monkey, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human HSP90B around the phosphorylation site of Ser254. AA range:226-275
• Alternative Names: HSP90AB1, HSP90B, HSPC2, HSPCB, Heat shock protein HSP 90-beta, HSP 90, Heat shock 84 kDa, HSP 84, HSP84

Product Description

This gene encodes a member of the heat shock protein 90 family, these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 90kD
• NCBI: 3326
• UniProt: P08238

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

Images

Western Blot analysis of various cells using Phospho-HSP90beta (S254) Polyclonal Antibody

Western Blot analysis of HepG2 cells using Phospho-HSP90beta (S254) Polyclonal Antibody

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HSP90B (Phospho-Ser254) Antibody