Crk-L (phospho Tyr207) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: Crk-L (phospho Tyr207) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1517
• Supplier Catalog Number: ES1517
• Alternative Catalog Number: EBT-ES1517-50, EBT-ES1517-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Monkey, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human CrkL around the phosphorylation site of Tyr207. AA range:173-222
• Alternative Names: CRKL, Crk-like protein

Product Description

This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 39kD
• NCBI: 1399
• UniProt: P46109

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

Images

Western Blot analysis of various cells using Phospho-Crk-L (Y207) Polyclonal Antibody

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunofluorescence analysis of HUVEC cells, using CrkL (Phospho-Tyr207) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using CrkL (Phospho-Tyr207) Antibody. The picture on the right is blocked with the phospho peptide.