14-3-3 zeta rabbit pAb, Rabbit, Polyclonal

Catalog Number: EBT-ES1543
Article Name: 14-3-3 zeta rabbit pAb, Rabbit, Polyclonal
Biozol Catalog Number: EBT-ES1543
Supplier Catalog Number: ES1543
Alternative Catalog Number: EBT-ES1543-50, EBT-ES1543-100
Manufacturer: ELK Biotechnology
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, IHC, IP, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: The antiserum was produced against synthesized peptide derived from human 14-3-3 zeta. AA range:24-73
Alternative Names: YWHAZ, 14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5 UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008],
Clonality: Polyclonal
Concentration: 1 mg/ml
Molecular Weight: 28kD
NCBI: 7534
UniProt: P63104
Application Dilute: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Western Blot analysis of various cells using 14-3-3 zeta Polyclonal Antibody diluted at 1:1000
Western Blot analysis of COLO205 cells using 14-3-3 zeta Polyclonal Antibody diluted at 1:1000
Western Blot analysis of 293T HELA using 14-3-3 zeta Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog:RS0002) was diluted at 1:20000
Immunofluorescence analysis of HeLa cells, using 14-3-3 zeta Antibody. The picture on the right is blocked with the synthesized peptide.