ACOT4 rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: ACOT4 rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1584
• Supplier Catalog Number: ES1584
• Alternative Catalog Number: EBT-ES1584-50, EBT-ES1584-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human ACOT4. AA range:361-410
• Alternative Names: ACOT4, PTE2B, PTEIB, Acyl-coenzyme A thioesterase 4, Acyl-CoA thioesterase 4, PTE-2b, Peroxisomal acyl coenzyme A thioester hydrolase Ib, Peroxisomal long-chain acyl-CoA thioesterase Ib, PTE-Ib

Product Description

catalytic activity:Palmitoyl-CoA + H(2)O = CoA + palmitate.,function:Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH.,similarity:Belongs to the C/M/P thioester hydrolase family.,

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 48kD
• NCBI: 122970
• UniProt: Q8N9L9

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Images

Western Blot analysis of various cells using ACOT4 Polyclonal Antibody diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4 overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunofluorescence analysis of MCF7 cells, using ACOT4 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HeLa and COLO cells, using ACOT4 Antibody. The lane on the right is blocked with the synthesized peptide.