The antiserum was produced against synthesized peptide derived from human AF10. AA range:211-260
Alternative Names:
MLLT10, AF10, Protein AF-10, ALL1-fused gene from chromosome 10 protein
myeloid/lymphoid or mixed-lineage leukemia, translocated to, 10(MLLT10) Homo sapiens This gene encodes a transcription factor and has been identified as a partner gene involved in several chromosomal rearrangements resulting in various leukemias. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010],
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Immunofluorescence analysis of Hela cell. 1,AF-10 Polyclonal Antibody(red) was diluted at 1:200(4 overnight). COX IV Monoclonal Antibody(6C8)(green) was diluted at 1:200(4 overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
Western Blot analysis of various cells using AF-10 Polyclonal Antibody diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Western blot analysis of lysates from COLO205 and Jurkat cells, using AF10 Antibody. The lane on the right is blocked with the synthesized peptide.
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