The antiserum was produced against synthesized peptide derived from human AIM2. AA range:51-100
Alternative Names:
AIM2, Interferon-inducible protein AIM2, Absent in melanoma 2
AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2. [provided by RefSeq, Jul 2008],
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Immunofluorescence analysis of Hela cell. 1,AIM2 Polyclonal Antibody(red) was diluted at 1:200(4 overnight). HSP70 Monoclonal Antibody(3G10)(green) was diluted at 1:200(4 overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
Western blot analysis of lysates from 1) HuvEc, 2) Jurkat cells, (Green) primary antibody was diluted at 1:1000, 4over night, Dylight 800 secondary antibody(Immunoway:RS23920)was diluted at 1:10000, 37 1hour. (Red) Tubulin beta Monoclonal Antibody(5G3) (Immunoway:YM3030) antibody was diluted at 1:5000 as loading control, 4 over night,Dylight 680 secondary antibody(Immunoway:RS23710)was diluted at 1:10000, 37 1hour.
Western Blot analysis of HuvEc cells using AIM2 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Western blot analysis of lysates from HUVEC cells, using AIM2 Antibody. The lane on the right is blocked with the synthesized peptide.
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