The antiserum was produced against synthesized peptide derived from human SLC25A31. AA range:131-180
Alternative Names:
SLC25A31, AAC4, ANT4, SFEC, ADP/ATP translocase 4, ADP, ATP carrier protein 4, Adenine nucleotide translocator 4, ANT 4, Solute carrier family 25 member 31, Sperm flagellar energy carrier protein
The protein encoded by this gene is a member of the ADP/ATP carrier family of proteins that exchange cytosolic ADP for matrix ATP in the mitochondria. Cells over-expressing this gene have been shown to display an anti-apoptotic phenotype. This protein is also thought to play a role in spermatogenesis, where it is believed to associate with a part of the flagellar cytoskeleton and with glycolytic enzymes. Male mice with mutations in the mouse ortholog of this gene are sterile and spermatocytes display an early meiotic arrest phenotype. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016],
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Western Blot analysis of various cells using ANT4 Polyclonal Antibody diluted at 1:500
Western blot analysis of lysates from HeLa, HUVEC, and COLO cells, using SLC25A31 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from K562 cells using SLC25A31 antibody.
Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4 overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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