APHC rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: APHC rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1677
• Supplier Catalog Number: ES1677
• Alternative Catalog Number: EBT-ES1677-50, EBT-ES1677-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse
• Immunogen: The antiserum was produced against synthesized peptide derived from human PHCA. AA range:171-220
• Alternative Names: ACER3, APHC, PHCA, Alkaline ceramidase 3, AlkCDase 3, Alkaline CDase 3, Alkaline dihydroceramidase SB89, Alkaline phytoceramidase, aPHC

Product Description

enzyme regulation:Activated by Ca(2+) and inhibited by Zn(2+).,function:Hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity.,similarity:Belongs to the alkaline ceramidase family.,tissue specificity:Ubiquitously expressed. Highest expression in placenta.,

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 35kD
• NCBI: 55331
• UniProt: Q9NUN7

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

Images

Western Blot analysis of various cells using APHC Polyclonal Antibody

Immunofluorescence analysis of MCF7 cells, using PHCA Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human placenta tissue, using PHCA Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from COLO cells, using PHCA Antibody. The lane on the right is blocked with the synthesized peptide.