API5 rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: API5 rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1678
• Supplier Catalog Number: ES1678
• Alternative Catalog Number: EBT-ES1678-50, EBT-ES1678-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse
• Immunogen: The antiserum was produced against synthesized peptide derived from human API-5. AA range:421-470
• Alternative Names: API5, MIG8, Apoptosis inhibitor 5, API-5, Antiapoptosis clone 11 protein, AAC-11, Cell migration-inducing gene 8 protein, Fibroblast growth factor 2-interacting factor, FIF, Protein XAGL

Product Description

This gene encodes an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates Acinus, a nuclear factor involved in apoptotic DNA fragmentation. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 58kD
• NCBI: 8539
• UniProt: Q9BZZ5

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Images

Western Blot analysis of various cells using API5 Polyclonal Antibody diluted at 1:1000

Western Blot analysis of HuvEc cells using API5 Polyclonal Antibody diluted at 1:1000

Immunohistochemistry analysis of paraffin-embedded human heart tissue, using API-5 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293, Jurkat, and HUVEC cells, using API-5 Antibody. The lane on the right is blocked with the synthesized peptide.