The antiserum was produced against synthesized peptide derived from human ATP5I. AA range:20-69
Alternative Names:
ATP5I, ATP5K, ATP synthase subunit e, mitochondrial, ATPase subunit e
Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the e subunit of the Fo complex. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jun 2010],
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Western Blot analysis of various cells using ATP5I Polyclonal Antibody
Western blot analysis of lysates from COLO cells, using ATP5I Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from 293 cells using ATP5I antibody.
Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200(4 overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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