The antiserum was produced against synthesized peptide derived from human ZNF174. AA range:201-250
Alternative Names:
ZNF174, ZSCAN8, Zinc finger protein 174, AW-1, Zinc finger and SCAN domain-containing protein 8
function:Transcriptional repressor.,similarity:Belongs to the krueppel C2H2-type zinc-finger protein family.,similarity:Contains 1 SCAN box domain.,similarity:Contains 3 C2H2-type zinc fingers.,subunit:Homodimer.,tissue specificity:Expressed in a variety of organs, but most strongly in adult testis and ovary followed by small intestine, colon, prostate, thymus, spleen, pancreas, skeletal muscle, heart, brain and kidney. Also expressed in umbilical vein endothelial cells, foreskin fibroblast and HEPG2 cells.,
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Western Blot analysis of various cells using AW-1 Polyclonal Antibody
Immunofluorescence analysis of HUVEC cells, using ZNF174 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HeLa cells, using ZNF174 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4 overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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