The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in this gene are associated with Crouzon syndrome, Pfeiffer syndrome, C
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Immunofluorescence analysis of Hela cell. 1,Bek Polyclonal Antibody(green) was diluted at 1:200(4 overnight). (red) was diluted at 1:200(4 overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog:RS3608 was diluted at 1:1000(room temperature, 50min).
Western blot analysis of lysates from 1) A549 , 2) HuvEc, 3) HepG2 cells, (Green) primary antibody was diluted at 1:1000, 4over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37 1hour. (Red) Tubulin beta Monoclonal Antibody(5G3) (cat:YM3030) antibody was diluted at 1:5000 as loading control, 4 over night,secondary antibody(cat:RS23710)was diluted at 1:10000, 37 1hour.
Western Blot analysis of various cells using Bek Polyclonal Antibody
Western Blot analysis of A549 cells using Bek Polyclonal Antibody
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