BIG2 rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: BIG2 rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1770
• Supplier Catalog Number: ES1770
• Alternative Catalog Number: EBT-ES1770-50, EBT-ES1770-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human ARFGEF2. AA range:1491-1540
• Alternative Names: ARFGEF2, ARFGEP2, BIG2, Brefeldin A-inhibited guanine nucleotide-exchange protein 2, Brefeldin A-inhibited GEP 2, ADP-ribosylation factor guanine nucleotide-exchange factor 2

Product Description

ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP and is involved in Golgi transport. It contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition. [provided by RefSeq, Jul 2008],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 210kD
• NCBI: 10564
• UniProt: Q9Y6D5

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Images

Western Blot analysis of various cells using BIG2 Polyclonal Antibody

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4 overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4 overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunofluorescence analysis of A549 cells, using ARFGEF2 Antibody. The picture on the right is blocked with the synthesized peptide.