The antiserum was produced against synthesized peptide derived from human BUB3. AA range:279-328
Alternative Names:
BUB3, Mitotic checkpoint protein BUB3
This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008],
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Western Blot analysis of various cells using Bub3 Polyclonal Antibody
Western Blot analysis of 293 cells using Bub3 Polyclonal Antibody
Western blot analysis of lysates from HeLa cells, Jurkat cells, and 293 cells, using BUB3 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4 overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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