eIF2alpha rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: eIF2alpha rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES2244
• Supplier Catalog Number: ES2244
• Alternative Catalog Number: EBT-ES2244-50, EBT-ES2244-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Fish, Human, Monkey, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human eIF2 alpha. AA range:21-70
• Alternative Names: EIF2S1, EIF2A, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha

Product Description

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2, MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 38kD
• NCBI: 1965
• UniProt: P05198

Application Notes

• Application Dilute: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Images

Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.