Oct-2 rabbit pAb, Rabbit, Polyclonal

Catalog Number: EBT-ES3001
Article Name: Oct-2 rabbit pAb, Rabbit, Polyclonal
Biozol Catalog Number: EBT-ES3001
Supplier Catalog Number: ES3001
Alternative Catalog Number: EBT-ES3001-50, EBT-ES3001-100
Manufacturer: ELK Biotechnology
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, IHC, WB
Species Reactivity: Human, Monkey, Mouse
Immunogen: The antiserum was produced against synthesized peptide derived from human OCT2. AA range:1-50
Alternative Names: POU2F2, OCT2, OTF2, POU domain, class 2, transcription factor 2, Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2, Octamer-binding protein 2, Oct-2, Octamer-binding transcription factor 2, OTF-2
The protein encoded by this gene is a homeobox-containing transcription factor of the POU domain family. The encoded protein binds the octamer sequence 5-ATTTGCAT-3, a common transcription factor binding site in immunoglobulin gene promoters. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011],
Clonality: Polyclonal
Concentration: 1 mg/ml
Molecular Weight: 52kD
NCBI: 5452
UniProt: P09086
Application Dilute: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Western Blot analysis of various cells using Oct-2 Polyclonal Antibody
Immunofluorescence analysis of COS7 cells, using OCT2 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from COS7 cells, using OCT2 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human brain tumor. 1, Antibody was diluted at 1:200(4 overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).