The antiserum was produced against synthesized peptide derived from human RHBT2. AA range:61-110
Alternative Names:
RHOBTB1, KIAA0740, Rho-related BTB domain-containing protein 1, RHOBTB2, DBC2, KIAA0717, Rho-related BTB domain-containing protein 2, Deleted in breast cancer 2 gene protein, p83
The protein encoded by this gene belongs to the Rho family of the small GTPase superfamily. It contains a GTPase domain, a proline-rich region, a tandem of 2 BTB (broad complex, tramtrack, and bric-a-brac) domains, and a conserved C-terminal region. The protein plays a role in small GTPase-mediated signal transduction and the organization of the actin filament system. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008],
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Western Blot analysis of various cells using RhoBTB1/2 Polyclonal Antibody
Western blot analysis of lysates from HepG2 and HeLa cells, using RHBT2 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4 overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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