The antiserum was produced against synthesized peptide derived from human WDHD1. AA range:721-770
Alternative Names:
WDHD1, AND1, WD repeat and HMG-box DNA-binding protein 1, Acidic nucleoplasmic DNA-binding protein 1, And-1
The protein encoded by this gene contains multiple N-terminal WD40 domains and a C-terminal high mobility group (HMG) box. WD40 domains are found in a variety of eukaryotic proteins and may function as adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly. HMG boxes are found in many eukaryotic proteins involved in chromatin assembly, transcription and replication. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Western Blot analysis of various cells using WDHD1 Polyclonal Antibody. Secondary antibody(catalog:RS0002) was diluted at 1:20000
Western blot analysis of lysates from Jurkat and HepG2 cells, using WDHD1 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4 overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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