VE-Cadherin (phospho Tyr731) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: VE-Cadherin (phospho Tyr731) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES4410
• Supplier Catalog Number: ES4410
• Alternative Catalog Number: EBT-ES4410-50, EBT-ES4410-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse
• Immunogen: The antiserum was produced against synthesized peptide derived from human VE-Cadherin around the phosphorylation site of Tyr731. AA range:697-746
• Alternative Names: CDH5, Cadherin-5, 7B4 antigen, Vascular endothelial cadherin, VE-cadherin, CD antigen CD144

Product Description

This gene encodes a classical cadherin of the cadherin superfamily. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion molecule is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classical cadherin by imparting to cells the ability to adhere in a homophilic manner, this protein plays a role in endothelial adherens junction assembly and maintenance. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. [provided by RefSeq, Nov 2015],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 130kD
• NCBI: 1003
• UniProt: P33151

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Images

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using VE-Cadherin (Phospho-Tyr731) Antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using VE-Cadherin (Phospho-Tyr731) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HepG2 cells treated with Na3VO4 0.3mM 40, using VE-Cadherin (Phospho-Tyr731) Antibody. The lane on the right is blocked with the phospho peptide.