Chk1 (phospho Ser280) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: Chk1 (phospho Ser280) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES4591
• Supplier Catalog Number: ES4591
• Alternative Catalog Number: EBT-ES4591-50, EBT-ES4591-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human Chk1 around the phosphorylation site of Ser280. AA range:251-300
• Alternative Names: CHEK1, CHK1, Serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase-1

Product Description

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 54kD
• NCBI: 1111
• UniProt: O14757

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

Images

Western Blot analysis of 293T cells using Phospho-Chk1 (S280) Polyclonal Antibody diluted at 1:500

Western blot analysis of lysates from HeLa cells treated with Hu 2nM 24hours, using Chk1 (Phospho-Ser280) Antibody. The lane on the right is blocked with the phospho peptide.