c-Kit (phospho Tyr936) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: c-Kit (phospho Tyr936) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES6021
• Supplier Catalog Number: ES6021
• Alternative Catalog Number: EBT-ES6021-50, EBT-ES6021-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse
• Immunogen: The antiserum was produced against synthesized peptide derived from human KIT around the phosphorylation site of Tyr936. AA range:906-955
• Alternative Names: KIT, SCFR, Mast/stem cell growth factor receptor Kit, SCFR, Piebald trait protein, PBT, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, p145 c-kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, CD antigen CD117

Product Description

This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 145kD
• NCBI: 3815
• UniProt: P10721

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Images

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4 overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemistry analysis of paraffin-embedded human heart, using KIT (Phospho-Tyr936) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30, using KIT (Phospho-Tyr936) Antibody. The lane on the right is blocked with the phospho peptide.