Rb (phospho Ser249) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: Rb (phospho Ser249) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES7000
• Supplier Catalog Number: ES7000
• Alternative Catalog Number: EBT-ES7000-50, EBT-ES7000-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human Retinoblastoma around the phosphorylation site of Ser249. AA range:221-270
• Alternative Names: RB1, Retinoblastoma-associated protein, p105-Rb, pRb, Rb, pp110

Product Description

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• NCBI: 5925
• UniProt: P06400

Application Notes

• Application Dilute: WB 1:500-2000,IHC-p 1:50-300, ELISA 2000-20000

Images

Immunofluorescence analysis of HeLa cells, using Retinoblastoma (Phospho-Ser249) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200(4 overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).