p130 Cas (phospho Tyr249) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: p130 Cas (phospho Tyr249) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES8062
• Supplier Catalog Number: ES8062
• Alternative Catalog Number: EBT-ES8062-50, EBT-ES8062-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human p130 Cas around the phosphorylation site of Tyr249. AA range:215-264
• Alternative Names: BCAR1, CAS, CASS1, CRKAS, Breast cancer anti-estrogen resistance protein 1, CRK-associated substrate, Cas scaffolding protein family member 1, p130cas

Product Description

BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM, May 2009],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 130kD
• NCBI: 9564
• UniProt: P56945

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Images

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using p130 Cas (Phospho-Tyr249) Antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p130 Cas (Phospho-Tyr249) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30 and A549 cells treated with PMA 125ng/ml 30, using p130 Cas (Phospho-Tyr249) Antibody. The lane on the right is blocked with the phospho peptide.