Ac-Asp-Glu-Val-Asp-pNA (Caspase-3 (Apopain) Substrate)

Product Overview

• Article Name: Ac-Asp-Glu-Val-Asp-pNA (Caspase-3 (Apopain) Substrate)
• Biozol Catalog Number: ECH-860-20
• Supplier Catalog Number: 860-20
• Alternative Catalog Number: ECH-860-20-25MG, ECH-860-20-5MG
• Manufacturer: Echelon Biosciences
• Category: Molekularbiologie

Product Description

CAS Number: 189950-66-1 Molecular Weight: 638.22 Salt Form: TFA Purity: >96% Sequence (3-letter): Ac-Asp-Glu-Val-Asp-pNA Sequence (1-letter): Ac-DEVD-pNA Storage: -20 ?C or below Solubility: DMSO and acetonitrile:methanol (1:1) to 1 mg/ml Caspase-3 (Apopain) Substrate is a colorimetric substrate for caspase-3 with the sequence Ac-DEVD-pNA. Caspase-3 is a protease that is rapidly activated during apoptosis and that cleaves several substrates, including poly(ADP-ribose) polymerase (PARP). Cleavage of Ac-DEVD-pNA is monitored by measuring absorbance at 405 nm. Ac-DEVD-pNA is poorly cleaved by caspase-2. Km = 18 ?M (Casp-1), 11 ?M (Casp-3), 32 ?M (Casp-4), 180 ?M (Casp-6), 12 ?M (Casp-7)

References

1. Talanian, R.V. et al. (1997) ?Substrate Specificities of Caspase Family Proteases? J. Biol. Chem. 272 (15): 9677?9682. 2.Nagane, M. et al. (1998) ?Drug resistance of human glioblastoma cells conferred by a tumor-specific mutant epidermal growth factor receptor through modulation of Bcl-XL and caspase-3-like proteases? Proc. Natl. Acad. Sci. USA 95 (10): 5724?5729.

Application Notes

• Application Notes: Categories: Peptides. Related: Caspase, Enzyme Substrates. Ship 1 : Shipping Temp. Ship 2: Ambient Temperature