Caspase 3 Substrate, fluorogenic

Product Overview

• Article Name: Caspase 3 Substrate, fluorogenic
• Biozol Catalog Number: ECH-860-30
• Supplier Catalog Number: 860-30
• Alternative Catalog Number: ECH-860-30-5MG
• Manufacturer: Echelon Biosciences
• Category: Molekularbiologie

Product Description

Molecular Weight: 760 Salt Form: N/A Purity: >95% Sequence (3-letter): Ac-Asp-Met-Gln-Asp-AFC Sequence (1-letter): Ac-DMQD-AFC Storage: -20 ?C or below Ac-Asp-Met-Gln-Asp-AFC is a fluorogenic substrate for Caspase 3 (Apopain). Caspase 3 is a cysteine protease that is rapidly activated during apoptosis and that cleaves several substrates, including poly(ADP-ribose) polymerase (PARP). Caspase 3 is activated by Caspases 8, 9 and 10 and cleaves and activates caspase 6 and 7. Caspase 3 cleaves the amyloid precursor protein (APP) contributing to Amyloid beta formation and has been implicated in Alzheimer?s disease and proposed as a target for drug discovery. Activity is quantified by release of free fluorescent 7-amino-4-trifluoromethylcoumarin (AFC) which excites at 395-400 nm and emits at 495-505 nm.

References

1. Talanian, R.V. et al. (1997) ?Substrate Specificities of Caspase Family Proteases? J. Biol. Chem. 272 (15): 9677?9682. 2.Nagane, M. et al. (1998) ?Drug resistance of human glioblastoma cells conferred by a tumor-specific mutant epidermal growth factor receptor through modulation of Bcl-XL and caspase-3-like proteases? Proc. Natl. Acad. Sci. USA 95 (10): 5724?5729. 3) Troy T. Rohn, Elizabeth Head ?Caspases as Therapeutic Targets in Alzheimer?s Disease: Is It Time to ?Cut? to the Chase?? Int J Clin Exp Pathol. 2009, 2(2): 108?118.

Application Notes

• Application Notes: Categories: Peptides. Related: Caspase, Enzyme Substrates. Ship 1 : Shipping Temp. Ship 2: Ambient Temperature