Molecular Weight: 500.23Salt Form: N/APurity: >96%Sequence (3-letter): Ac-Val-Ala-Asp-4MbNASequence (1-letter): Ac-VAD-4MbNAStorage: -20 AC or belowAc-Val-Ala-Asp-4MbNA is a fluorogenic substrate for Caspase 1. Caspase 1/Interleukin 1b converting enzyme (ICE) is a cysteine protease that preferentially cleaves substrates built on VAD (val-ala-asp) or WEAD (trp-glu-ala-asp) sequences. ICE converts inactive IL-1 beta precursor to a pro-inflammatory cytokine that mediates a wide range of immune and inflammatory response. ICE-related cysteine proteases are required for E1A-induced p53-dependent apoptosis in baby rat kidney (BRK) cells. Adenovirus E1B 19K protein which is a potent inhibitor of apoptosis inhibits activation of these proteases in BRK cells.Activity is quantified by release of free fluorescent 4-Methoxy-i-naphthylamine (4MiNA) which excites at 335-350 nm and emits at 410-440 nm.
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1208 1179
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