Molecular Weight: 681.31Salt Form: AcetatePurity: >96%Sequence (3-letter): Boc-Val-Pro-Arg-AFCSequence (1-letter): Boc-VPR-AFCStorage: -20 AC or belowBoc-Val-Pro-Arg-AFC is a fluorogenic substrate for alpha-thrombin (Km = 21 iM kcat = 109 s-1) and the thrombin-staphylocoagulase complex (Km = 25 iM kcat = 89 s-1). The substrate is also cleaved by trypsin tryptase from rat mast cells Kex2 endoprotease and acrosin and spermosin (trypsin-like enzymes from sperm of the ascidian Halocynthia roretzi).Activity is quantified by release of free fluorescent 7-amino-4-trifluoromethylcoumarin (AFC) which excites at 395-400 nm and emits at 495-505 nm.
Tag:
1179
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