PTEN Enzyme, active

Product Overview

• Article Name: PTEN Enzyme, active
• Biozol Catalog Number: ECH-E-3000
• Supplier Catalog Number: E-3000
• Alternative Catalog Number: ECH-E-3000-10UG,ECH-E-3000-2.5UG
• Manufacturer: Echelon Biosciences
• Category: Molekularbiologie

Product Description

PTEN Enzyme, active is a N-Terminal GST-Tagged, recombinent human PTEN (Phosphatase and Tensin Homolog), 76kD, purified from E.coli using glutathione-sepharose column chromatography. Storage: Store product > -70 C. Enzyme is stable for at least 6 months at -70 C as undiluted stock Specificity: PTEN selectively removes phosphate from the 3 position of the inositol ring of PtdIns(3,4,5)P3 Specific Activity: request Certificate of Analysis (COA)

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Product Description The PTEN enzyme is a 3-phosphoinositide phosphatase that converts PI(3,4,5)P3 to PI(4,5)P2, thus opposing PKB/Akt activation by PI3-K. PTEN is involved in neuronal stem cell proliferation and self-renewal, cardiac myocyte hypertrophy and contractility, and a wide range of developmental processes. PTEN, however, is best known for its role as a tumor suppressor. Loss of PTEN activity results in accumulation of PI(3,4,5)P3, abnormal activation of PKB/Akt, unregulated cell growth, suppression of apoptosis, and increase tumorigenesis in a number of human tissues. It has also been proposed that PTEN is a candidate for targeted chemotherapy because certain anti-cancer agents preferentially destroy tumors with PTEN mutations. In addition to this direct role in cancer, PTEN also indirectly regulates cancer-associated pathways including VEGF-mediated angiogenesis among others. References 1. Brooks, R., et al. (2010). SHIP1 inhibition increases immunoregulatory capacity and triggers apoptosis of hematopoietic cancer cells. J Immunol 184(7): 3582-3589. 2. Caromile, L. A., et al. (2010). The neurosecretory vesicle protein phogrin functions as a phosphatidylinositol phosphatase to regulate insulin secretion. J Biol Chem 285(14): 10487-10496. 3. Thakral, C., et al. (2012). ALK+ anaplastic large cell lymphoma exhibits phosphatidylinositol-3 kinase/akt activity with retained but inactivated PTEN-A report from the Childrens Oncology Group. Pediatric Blood & Cancer 59(3): 440-447. 4. Steidle, E. A., et al. (2016). A novel inositol pyrophosphate phosphatase in Saccharomyces cerevisiae: Siw14 selectively cleaves the b-phosphate from 5-diphosphoinositol pentakisphosphate (5PP-IP5). J. Biol. Chem. 291: 6772-6783. 5. Alsina-Sanchis, E., et al. (2018). ALK1 Loss Results in Vascular Hyperplasia in Mice and Humans Through PI3K Activation. Arteriosclerosis, thrombosis, and vascular biology 38(5): 1216-1229.

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Application Notes

• Application Notes: Categories: Antibodies & Proteins. Related: Enzyme, Phosphatase, PTEN. Ship 1 : Shipping Temp. Ship 2: Dry Ice