5 PtdIns(3,4,5)P3 Phosphatase Activity Fluorescence Polarization Assay

Product Overview

• Article Name: 5 PtdIns(3,4,5)P3 Phosphatase Activity Fluorescence Polarization Assay
• Biozol Catalog Number: ECH-K-1400
• Supplier Catalog Number: K-1400
• Alternative Catalog Number: ECH-K-1400-1KIT
• Manufacturer: Echelon Biosciences
• Category: Molekularbiologie

Product Description

The 5 PtdIns(3,4,5)P3 Phosphatase Activity Fluorescence Polarization Assay determines activity through quantification of the end product, PI(3,4)P2. Sample Type: in vitro determination of 5 phosphatase activity in cancer tissues and cell lines and compound screening for drug discovery Sample Volume: 2.5-5.0 µL enzyme / inhibitor Assay Range: 0.0625 µM to 2.0 µM

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Product Background

This 384 well, fluorescence polarization-based, competitive assay measures the phosphatase activity of SHIP2 which hydrolyzes the 5-phosphate from PtdInd(3,4,5)P3. After the phosphatase reactions are complete, reaction products are mixed with a PI(3,4)P2 detector protein and the fluorescent PI(3,4)P2 probe. Polarization (mP) values decrease as probe binding to the PI(3,4)P2 detector is displaced by PI(3,4)P2 produced by enzymatic activity and the amount of unbound fluorescent probe in the mixture increases.

Featured in Publications

1. Drees, B. E., A. Weipert, et al. (2003). ''Competitive fluorescence polarization assays for the detection of phosphoinositide kinase and phosphatase activity. Comb Chem High Throughput Screen 6(4): 321-330. 2. Brooks, R., et al. (2010). SHIP1 inhibition increases immunoregulatory capacity and triggers apoptosis of hematopoietic cancer cells. J Immunol 184(7): 3582-3589. 3. Fuhler, G. M. B., R, Toms, Iyer, Geno (2012). Therapeutic potential of SHIP1 and SHIP2 inhibition in cancer cells. Molecular Medicine 18: 65-75. 4. Watt, N. T., et al. (2017). Endothelial SHIP2 Suppresses Nox2 NADPH Oxidase-Dependent Vascular Oxidative Stress, Endothelial Dysfunction and Systemic Insulin Resistance. Diabetes 66(11): 2808-2821. Note to Purchaser: Echelon Biosciences products are sold for research and development purposes only and are not to be incorporated into products for resale without written permission from Echelon Biosciences. This kit and all non-radioactive, competitive assays for determining phosphoinositide-3-kinase (PI3-K) activity are covered by Echelon Biosciences Inc. U.S. Patent 7,067,269. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchasers own internal research. For inquiries email busdev echelon-inc.com. Product Keywords: SHIP2, SHIP1, PI(3,4,)P2

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Documents

Technical Data Sheet

Application Notes

• Application Notes: Categories: Bioassays & Kits. Related: Activity, Inhibitor, Phosphatase, PI(3\,4\,5)P3. Ship 1 : Shipping Temp. Ship 2: Dry ice or gel ice depending on destination