CDP-MEP Synthesis Kit

Product Overview

• Article Name: CDP-MEP Synthesis Kit
• Biozol Catalog Number: ECH-K-2000E
• Supplier Catalog Number: K-2000E
• Alternative Catalog Number: ECH-K-2000E-1KIT
• Manufacturer: Echelon Biosciences
• Category: Molekularbiologie

Product Description

The CDP-MEP Synthesis Kit contains the components necessary for the synthesis of CDP-MEP. Sample Type: For Enzymatic Synthesis of CDP-MEP Assay Incubation time: Sixty minutes (CDP-MEP Synthesis Reaction) Sample Considerations: Yield 70% CDP-MEP (~ 0.7 mg) Sample Volume: 1.5 mL (0.7 mg CDP-ME)

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Product Background

In the fourth step of the MEP pathway, CDP-ME is converted to 4-diphosphocytidyl-2-C-methyl-D-erythritol-2-phosphate (CDP-MEP) by CDP-ME kinase (IspE) with the concomitant formation of adenosine diphosphate (ADP). The formation of CDP-MEP occurs at a 1:1 ratio with the consumption of ATP. By measuring this decrease in ATP levels the user can infer the amount of CDP-MEP generated. The user can expect 1,600 µL of >600 µM CDP-MEP produced (>60% yield).

Featured in Publications

1. Li, Z. and T. D. Sharkey (2012). Metabolic Profiling of the Methylerythritol Phosphate Pathway Reveals the Source of Post-illumination Isoprene Burst from Leaves. Plant, Cell & Environment.

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Documents

Technical Data Sheet

Application Notes

• Application Notes: Categories: Bioassays & Kits. Related: Isoprenoid, MEP Pathway. Ship 1 : Shipping Temp. Ship 2: Dry ice or gel ice depending on destination