Sphingosine Kinase Activity Assay
Catalog Number:
ECH-K-3500
| Article Name: |
Sphingosine Kinase Activity Assay |
| Biozol Catalog Number: |
ECH-K-3500 |
| Supplier Catalog Number: |
K-3500 |
| Alternative Catalog Number: |
ECH-K-3500-1KIT |
| Manufacturer: |
Echelon Biosciences |
| Category: |
Molekularbiologie |
The Sphingosine Kinase Activity Assay is a luminescent assay that measures sphingosine kinase activity by detecting the decrease of ATP. Sample Type: purified enzyme, cell lysate/homogenate Sample Volume: 10 µL / sample (duplicate points) Assay Incubation Time: 30 minutes to 2 hours
Product Background The Sphingosine Kinase Activity Assay is prepared with the addition of sphingosine kinase, sphingosine (substrate) in reaction buffer, and initiated by the addition of ATP. The reaction is stopped by addition of the ATP detector at the chosen reaction time. The luminescent signal is inversely correlated with the kinase activity. The sphingosine kinase activity assay was tested with Echelons SPHK1 and SPHK2. Sphingosine kinases (SPHKs) catalyze the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P). SPHKs have been implicated in proliferation, survival, migration and regulation of Ca2+ homoeostasis, development and regulation of the cardiovascular and nervous systems, inflammation, immunity, and cancer growth. There are two currently known SPHK isoforms, SPHK1 and SPHK2. It is believed that SPHK1 and SPHK2 have opposite functions, promoting cell growth and apoptosis respectively. Featured in Publications 1. Li, QF., Zhu, HY. et al. ''Prokineticin-1/endocrine gland-derived vascular endothelial growth factor is a survival factor for human multiple myeloma cells. Leuk Lymphoma 51:10 2. Cho, SY., Lee, HJ. et al. ''Sphingosine kinase 1 pathway is involved in melatonin-induced HIF-1 inactivation in hypoxic PC-3 prostate cancer cells. J Pineal Res 51:1 3.Guan, H., Song, L. et al. ''Sphingosine kinase 1 regulates the Akt/FOXO3a/Bim pathway and contributes to apoptosis resistance in glioma cells. PLoS One 6: 5 Product Keywords: Sphingosine kinase, SPHK, SK, Sphingosine kinase-1, SPHK1 enzyme, SK1, Sphingosine kinase type 2, SPHK2, SK2
|
| Application Notes: |
Categories: Bioassays & Kits. Related: Activity, Kinase. Ship 1 : Shipping Temp. Ship 2: Dry ice or gel ice depending on destination |
