The Autotaxin Activity Assay gives a quantitative measurement of autotaxin (ATX) activity in biological samples by measuring changes in fluorescence over time. Sample Volume: 400 µL / sample (50 µL per well, 8 wells per sample) Sample Number: 10 samples (in duplicate) / 96 well assay Sample Type: Currently this kit has been tested for the quantification of autotaxin activity in serum, plasma, ovarian ascites, and cell culture media.
Product Background
The Autotaxin Activity Assay, developed by Echelon Biosciences Inc., utilizes the fluorogenic autotaxin substrate, FS-3. FS-3 is an LPC analogue that is conjugated with both a fluorophore and a quencher. In its native state the quencher interferes with the fluorophores fluorescence however, once autotaxin cleaves FS-3, the fluorophore becomes liberated from the quencher, resulting in increased fluorescence. This assay gives a quantitative measurement of autotaxin activity in biological samples by measuring changes in fluorescence over time. Because the assay is not end point based, it has the advantage of being less susceptible to error arising from mistiming the addition of components to different sample wells. The final readout is in quantitative units that correct for inconsistencies between different users equipment.
